Question 1 of 15

Biology

1. A cDNA clone contains:

1.Exons

2.Anticodon

3.Introns

4.mRNA

Question 1 of 15

Question 2 of 15

2. A Plasmid:

1.Is a type of bacteriophage

2.Is a type of cDNA

3.Is used as DNA vector

4.Is a retrovirus

Question 2 of 15

Question 3 of 15

3. A Genomic DNA library:

1.Is stored in a collection of recombinant bacteria

2.Is a DNA copy of mature mRNAs.

3.Is made using reverse transcriptase

4.Represents all the DNA in a specific chromosome

Question 3 of 15

Question 4 of 15

4. The PCR technique polymerase chain reaction:

1.DNA ligase

2.Restriction enzymes

3.Heat resistance DNA polymerase

4.Reverse transcriptase

Question 4 of 15

Question 5 of 15

5. The Ti plasmid carried by Agrobacterium tumefaciens, is especially useful for introducing genes into a:

1.Plant

2.Bacteria

3.Animals

4.Yeast

Question 5 of 15

Question 6 of 15

6. A paleontologist, has recovered a bit of tissue from the 400 year old preserved skin of an extinct bird. The researcher would like to compare DNA from the sample with DNA from living birds. Which of the following would be most useful for increasing the amount of the bird DNA available for testing?

1.a RFLP analysis

2.polymerase chain reaction (PCR)

3.electroporation

4.southern hybridization

Question 6 of 15

Question 7 of 15

7. Plants are more readily manipulated by genetic engineering than are animals because:

1.Plant cell have large nuclei

2.Genes can be inserted into plant cells by microinjection

3.A somatic plant cell can often give rise to a complete plant

4.Plants genes do not contain interon.

Question 7 of 15

Question 8 of 15

8. The Dideoxynucleotides (ddATP, ddTTP, ddGTP, and ddCTP) are improvement in DNA sequencing because they:

1.Are used as prime

2.Are not affected by high temperature

3.Cause premature termination of growing DNA strand

4.Cause the DNA fragments that contain them to migrate more slowly through the gel

Question 8 of 15

Question 9 of 15

9. Which of the following sequences in double-stranded DNA is mostly likely to be recognized as a cutting site for a restriction enzyme?

1.AGTC TCAG

2.GGCC CCGG

3.AAGG TTCC

4.AC CA TGGT

Question 9 of 15

Question 10 of 15

10. Gel electrophoresis separates nucleic acid on the basis of difference:

1.Relative proportion of adenine and guanine

2.Nucleotide sequence

3.Charge

4.Length (molecular weight)

Question 10 of 15

Question 11 of 15

11. Which of the following tools of recombinant DNA technology is incorrectly paired with its use?

1.Restriction enzyme ------ production of RFLPs.

2.Reverse transcriptase ------ production of cDNA from mRNA.

3.Electrophoresis ------ separation of DNA fragments

4.DNA Ligase ----- enzyme that cuts DNA, creating the sticky ends of restriction fragments

Question 11 of 15

Question 12 of 15

12. Which technique rapidly replicates specific DNA fragments without cloning?

1.polymerase chain reaction

2.genetic probe

3.Gel electrophoresis

4.cDNA libraries

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Question 13 of 15

13. If eukaryotic DNA contains five cleavage sites for a particular restriction enzyme, how many fragments will be produced upon complete digestion of the DNA with that enzyme:

1.6

2.2

3.4

4.None

Question 13 of 15

Question 14 of 15

14. DNA molecules with complementary sticky ends associate by:

1.Ionic bond

2.Hydrogen bond

3.Disulphide bond

4.Covalent bond

Question 14 of 15

Question 15 of 15

15. Which of the following is highly polymorphic molecular marker which is useful in DNA fingerprinting?

1.A short tandeam repeats

2.Cosmid cloning vector

3.Palindromic DNA sequence

4.Cloned DNA sequence

Question 15 of 15

Test By Subject	Punjab
Test By Topics	12th Class Biology

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Punjab 12th Class Biology Chapter 23 Biotechnology Mcqs Test Online

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Subject

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Punjab 12th Class Biology Chapter 23 Biotechnology Mcqs Test Online

Biology Chapter 23 Biotechnology Mcqs Test Online Punjab Boards 12th Class

Biology

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