Question 1 of 15

Biology

1. If eukaryotic DNA contains five cleavage sites for a particular restriction enzyme, how many fragments will be produced upon complete digestion of the DNA with that enzyme:

1.4

2.None

3.2

4.6

Question 1 of 15

Question 2 of 15

2. Which technique rapidly replicates specific DNA fragments without cloning?

1.polymerase chain reaction

2.Gel electrophoresis

3.genetic probe

4.cDNA libraries

Question 2 of 15

Question 3 of 15

3. A Plasmid:

1.Is a type of cDNA

2.Is used as DNA vector

3.Is a retrovirus

4.Is a type of bacteriophage

Question 3 of 15

Question 4 of 15

4. Gel electrophoresis separates nucleic acid on the basis of difference:

1.Nucleotide sequence

2.Relative proportion of adenine and guanine

3.Charge

4.Length (molecular weight)

Question 4 of 15

Question 5 of 15

5. DNA molecules with complementary sticky ends associate by:

1.Ionic bond

2.Hydrogen bond

3.Covalent bond

4.Disulphide bond

Question 5 of 15

Question 6 of 15

6. A cDNA clone contains:

1.Anticodon

2.Introns

3.mRNA

4.Exons

Question 6 of 15

Question 7 of 15

7. A Genomic DNA library:

1.Is made using reverse transcriptase

2.Is a DNA copy of mature mRNAs.

3.Represents all the DNA in a specific chromosome

4.Is stored in a collection of recombinant bacteria

Question 7 of 15

Question 8 of 15

8. A paleontologist, has recovered a bit of tissue from the 400 year old preserved skin of an extinct bird. The researcher would like to compare DNA from the sample with DNA from living birds. Which of the following would be most useful for increasing the amount of the bird DNA available for testing?

1.polymerase chain reaction (PCR)

2.electroporation

3.a RFLP analysis

4.southern hybridization

Question 8 of 15

Question 9 of 15

9. Which of the following sequences in double-stranded DNA is mostly likely to be recognized as a cutting site for a restriction enzyme?

1.AC CA TGGT

2.AGTC TCAG

3.AAGG TTCC

4.GGCC CCGG

Question 9 of 15

Question 10 of 15

10. The PCR technique polymerase chain reaction:

1.DNA ligase

2.Restriction enzymes

3.Heat resistance DNA polymerase

4.Reverse transcriptase

Question 10 of 15

Question 11 of 15

11. Which of the following is highly polymorphic molecular marker which is useful in DNA fingerprinting?

1.Cosmid cloning vector

2.A short tandeam repeats

3.Palindromic DNA sequence

4.Cloned DNA sequence

Question 11 of 15

Question 12 of 15

12. Plants are more readily manipulated by genetic engineering than are animals because:

1.Genes can be inserted into plant cells by microinjection

2.Plant cell have large nuclei

3.A somatic plant cell can often give rise to a complete plant

4.Plants genes do not contain interon.

Question 12 of 15

Question 13 of 15

13. Which of the following tools of recombinant DNA technology is incorrectly paired with its use?

1.DNA Ligase ----- enzyme that cuts DNA, creating the sticky ends of restriction fragments

2.Electrophoresis ------ separation of DNA fragments

3.Restriction enzyme ------ production of RFLPs.

4.Reverse transcriptase ------ production of cDNA from mRNA.

Question 13 of 15

Question 14 of 15

14. The Dideoxynucleotides (ddATP, ddTTP, ddGTP, and ddCTP) are improvement in DNA sequencing because they:

1.Are used as prime

2.Cause the DNA fragments that contain them to migrate more slowly through the gel

3.Are not affected by high temperature

4.Cause premature termination of growing DNA strand

Question 14 of 15

Question 15 of 15

15. The Ti plasmid carried by Agrobacterium tumefaciens, is especially useful for introducing genes into a:

1.Plant

2.Yeast

3.Bacteria

4.Animals

Question 15 of 15

Test By Subject	Punjab
Test By Topics	12th Class Biology

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Punjab 12th Class Biology Chapter 23 Biotechnology Mcqs Test Online

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Punjab 12th Class Biology Chapter 23 Biotechnology Mcqs Test Online

Biology Chapter 23 Biotechnology Mcqs Test Online Punjab Boards 12th Class

Biology

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