Question 1 of 15

Biology

1. A Plasmid:

1.Is a retrovirus

2.Is used as DNA vector

3.Is a type of bacteriophage

4.Is a type of cDNA

Question 1 of 15

Question 2 of 15

2. If eukaryotic DNA contains five cleavage sites for a particular restriction enzyme, how many fragments will be produced upon complete digestion of the DNA with that enzyme:

1.None

2.6

3.4

4.2

Question 2 of 15

Question 3 of 15

3. Which of the following tools of recombinant DNA technology is incorrectly paired with its use?

1.DNA Ligase ----- enzyme that cuts DNA, creating the sticky ends of restriction fragments

2.Reverse transcriptase ------ production of cDNA from mRNA.

3.Restriction enzyme ------ production of RFLPs.

4.Electrophoresis ------ separation of DNA fragments

Question 3 of 15

Question 4 of 15

4. Which of the following is highly polymorphic molecular marker which is useful in DNA fingerprinting?

1.Palindromic DNA sequence

2.Cloned DNA sequence

3.Cosmid cloning vector

4.A short tandeam repeats

Question 4 of 15

Question 5 of 15

5. The Dideoxynucleotides (ddATP, ddTTP, ddGTP, and ddCTP) are improvement in DNA sequencing because they:

1.Are used as prime

2.Cause the DNA fragments that contain them to migrate more slowly through the gel

3.Cause premature termination of growing DNA strand

4.Are not affected by high temperature

Question 5 of 15

Question 6 of 15

6. A cDNA clone contains:

1.Anticodon

2.Exons

3.Introns

4.mRNA

Question 6 of 15

Question 7 of 15

7. A paleontologist, has recovered a bit of tissue from the 400 year old preserved skin of an extinct bird. The researcher would like to compare DNA from the sample with DNA from living birds. Which of the following would be most useful for increasing the amount of the bird DNA available for testing?

1.electroporation

2.southern hybridization

3.a RFLP analysis

4.polymerase chain reaction (PCR)

Question 7 of 15

Question 8 of 15

8. DNA molecules with complementary sticky ends associate by:

1.Hydrogen bond

2.Covalent bond

3.Disulphide bond

4.Ionic bond

Question 8 of 15

Question 9 of 15

9. Which of the following sequences in double-stranded DNA is mostly likely to be recognized as a cutting site for a restriction enzyme?

1.GGCC CCGG

2.AGTC TCAG

3.AC CA TGGT

4.AAGG TTCC

Question 9 of 15

Question 10 of 15

10. Plants are more readily manipulated by genetic engineering than are animals because:

1.Genes can be inserted into plant cells by microinjection

2.Plants genes do not contain interon.

3.Plant cell have large nuclei

4.A somatic plant cell can often give rise to a complete plant

Question 10 of 15

Question 11 of 15

11. The PCR technique polymerase chain reaction:

1.Reverse transcriptase

2.Heat resistance DNA polymerase

3.DNA ligase

4.Restriction enzymes

Question 11 of 15

Question 12 of 15

12. A Genomic DNA library:

1.Is made using reverse transcriptase

2.Represents all the DNA in a specific chromosome

3.Is stored in a collection of recombinant bacteria

4.Is a DNA copy of mature mRNAs.

Question 12 of 15

Question 13 of 15

13. Which technique rapidly replicates specific DNA fragments without cloning?

1.Gel electrophoresis

2.polymerase chain reaction

3.genetic probe

4.cDNA libraries

Question 13 of 15

Question 14 of 15

14. Gel electrophoresis separates nucleic acid on the basis of difference:

1.Relative proportion of adenine and guanine

2.Length (molecular weight)

3.Charge

4.Nucleotide sequence

Question 14 of 15

Question 15 of 15

15. The Ti plasmid carried by Agrobacterium tumefaciens, is especially useful for introducing genes into a:

1.Animals

2.Plant

3.Yeast

4.Bacteria

Question 15 of 15

Test By Subject	Punjab
Test By Topics	12th Class Biology

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Punjab 12th Class Biology Chapter 23 Biotechnology Mcqs Test Online

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Punjab 12th Class Biology Chapter 23 Biotechnology Mcqs Test Online

Biology Chapter 23 Biotechnology Mcqs Test Online Punjab Boards 12th Class

Biology

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